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All other F→M transsexuals had had regular menstrual cycles (28–31 days) before cross-sex hormone administration.

There was no evidence of autoimmune disease, immune deficiencies, chronic infection () was assessed, venous blood samples were taken in the morning between 0900–1200 h after an overnight fast, and 24-h urine was collected (during the 24 h before blood sampling).

The female predominance in several autoimmune diseases suggests a role for sex steroid hormones in disease susceptibility.

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T2 cells produce IL-4, IL-5, IL-10, and IL-13, which provide help for humoral immune responses (26, 27), such as the production of Ig E and Ig G4 (28, 29).

The body distribution of these T cells is directed by the differential expression on the cell membrane of distinct sets of adhesion molecules and chemokine receptors.

Chemokines are chemotactic cytokines produced by a wide variety of cells to attract the relevant leukocytes to sites of infection and inflammation (30, 31).

To assess peripheral androgen activity, we measured serum 5α-androstane-3α,17β-diol glucuronide (Adiol G) (43) by RIA (Diagnostic Systems Laboratories, Inc., Webster, TX).

Immunometric luminescence assays were used to determine levels of FSH (Amerlite, Amersham Pharmacia Biotech, Aylesbury, UK) and LH (Amerlite).

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