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Elucidating mechanism cellular uptake removal
The final colloidal solution was wine red with a gold concentration of ~ 0.5 m M.
The Au NP radius was also determined by ultraviolet and visible (UV-Vis) spectrophotometer (Shimadzu UV-3600).
Cell culture The osteoblast mouse cell line (MC3T3-E1) used in this study was obtained from the American Type Culture Collection (ATCC).
TEM grids were prepared by depositing a few drops of as prepared colloidal Au NP solution onto holey-carbon coated copper grids which were then allowed to dry for 15 minutes on filter paper.
X-ray energy dispersive spectroscopy (XEDS) was performed to confirm the composition of the Au NPs.
Received Date: September 03, 2011; Accepted November 04, 2011; Published Date: November 08, 2011 Citation: Mustafa T, Watanabe F, Monroe W, Mahmood M, Xu Y, et al.
(2011) Impact of Gold Nanoparticle Concentration on their Cellular Uptake by MC3T3-E1 Mouse Osteoblastic Cells as Analyzed by Transmission Electron Microscopy. doi:10.4172/2157-7439.1000118 Copyright: © 2011 Mustafa T, et al.A freshly prepared 50 ml of 1% trisodium citrate dehydrate was added to the solution, and the solution was kept at boiling temperature for an additional 20 min.It was then left to cool to room temperature; finally, the volume was adjusted to 500 ml by adding DI water.At the higher concentration (160 μg/ml), the particles seem to penetrate inside the cells primarily by endocytosis as the cells engulf Au NPs as agglomerates formed on the outer cellular membrane.At the lower concentrations of 10 μg/ml, Au NPs are more likely to cross the plasma membrane individually through diffusion.studies have indicated that Au NPs have the ability to distribute in different organs and tissues within the body ; and they can even penetrate subcellular organelles .In spite of the numerous studies mentioned above, a better understanding of Au NP interactions with cells is still needed.Gold nanoparticles; Uptake mechanism; MC3T3-E1 mouse osteocytic cells; Transmission electron microscopy Nanomaterials have great potential for use in biology and medicine given their unique properties, which enable them to be used as active agents in a number of applications ranging from drug [1-3] or gene-delivery [4-6] to tissue engineering.Investigations of the physical and chemical characteristics of nanomaterials that affect their uptake by eukaryotic cells can yield valuable information required for biomedical applications.The average size of the as-synthesized Au NPs used in this study was 12.2 (± 1.3) nm, and they were used to expose MC3T3-E1 osteoblastic cells at two concentrations (10 and 160 μg/ml) for 6, 24, and 96 hours before TEM imaging.Based on this analysis, we propose that the uptake mechanism of Au NPs is concentration-dependent.